Nanoprobes News

Current Issue

• EnzMet™:
  a Better and Simpler Stain for Immunohistochemistry (IHC)
• Newsflash:
  FDA Approves Breast Cancer Test Using EnzMet™
• On the Frontiers with EnzMet™:
  Featured Collaborators Drs. Ray Tubbs and Jim Pettay
• In the trenches, on the benches:
  Using EnzMet™ in Your Lab for Immunohistochemistry (IHC)
• Meetings and Courses with Nanoprobes:
  New Zealand Immunolabeling Workshop

 

Recent Issues

June/July, 2011: Volume 11, Number 6

• One probe, two answers:
  FluoroNanogold™ for Correlative Microscopy
• Dr. John Robinson
  Nanoprobes featured collaborator:
  On the frontiers with FluoroNanogold™
• In the Lab & in the Literature:
  FluoroNanogold™ in the study of tropical disease and HIV-related opportunistic infection
• Making correlative microscopy simple:
  FluoroNanogold™ Tips, Tricks and Protocols
• Upcoming Meetings and Courses
  Microscopy and Microanalysis 2011

Volume 11: 2011

May, 2011: Volume 11, Number 5

• Nanogold® for Electron Tomography:
  Revealing delicate structures in living tissue
• Dr. Wanzhong He
  Nanoprobes featured collaborator:
  On the frontiers with Nanogold®
• In the Lab & in the Literature:
  Cryo-electron Tomography with Nanogold®
• Hands-on with GoldEnhance™:
  When silver enhancement is not enough
  • GoldEnhance™ Tips, Tricks, and Protocols
• Upcoming Meetings and Courses

April, 2011: Volume 11, Number 4

• In Situ Hybridization:
  The evolution of detection, from Nanogold® to EnzMet™
• EnzMet™
  for in situ hybridization and immunohistochemistry:
  Features and Advantages
• Saving lives with EnzMet™: HER2 Cancer Test with SISH
  
• Other applications of EnzMet™
  • Electron Microscopy
  • Light and Electron Correlative Microscopy
  • Conductive Array Biochip Fabrication
• EnzMet™ Tips and Tricks
• Nanoprobes presenting at Microscopy & Microanalysis 2011

March, 2011: Volume 11, Number 3

• Seeing Gold Means Seeing More:
  Gold Nanoparticles as X-Ray Contrast Agents
• Can Gold Cure Cancer?
  Gold Nanoparticles in Radiation Therapy
• Upcoming Meetings:
  
  • American Association for Cancer Research 102nd Annual Meeting
    April 2-6, 2011: Orlando, FL, USA
  • SkyScan User Meeting
    April 12-15th, 2011: Leuven, Belgium
  • Microscopy & Microanalysis 2011
    August 7-11, 2011: Nashville, TN, USA

February 2011: Volume 11, Number 2

• Nanotechnology, Nanogold® and Nucleic Acids: Nanostructured Materials and Nanodevices
• Multifunctional Nanogold®:
• Positively Charged
• Negatively Charged

New Applications Articles:

• Fluoronanogold™ with larger gold nanoparticles
• Gold Labeling of Protein Fusion Tags for EM using 5nm Ni-NTA-Nanogold®

• New Paper:
  Nanogold® and Undecagold in nanostructured materials and nanodevices
• Visit Nanoprobes at
  Microscopy & Microanalysis 2011!

January 2011: Volume 11, Number 1

• Gold Labeling for Cryoelectron Microscopy and Electron Tomography
  
• Dealing with Disulfides:
  Alternatives to DTT or Mercaptoethylamine Reduction

 

Volume 10: 2009

• Nanogold^® In Situ Hybridization: Sensitive and Specific
• FluoroNanogold^TM: Updated Instructions, Tips and Tricks
• Alternative Combined Fluorescent and Gold Labeling: Nanogold^® with GFP
• AuroVist^TM: What is it and how do I use it?
  • How much should I use?
  • What instrument and beam settings should I use?
  • How can I ensure minimal toxicity?
• Nanoprobes Wins Another New SBIR Grant
• Other Recent Publications
  • Self-pressurized rapid freezing (SPRF) for cryo-EM
  • Gold nanoparticles for targeted delivery of beta-lapachone to tumors
  • X-ray microspectroscopic imaging and cellular speciation of biometals

• NTA-Ni(II)-Nanogold® Helps Assign 3-D Structure of Ca_v3.1
• Should I Use Nanogold®-Fab' or IgG?
• Inexpensive Conductimetric Biochips with EnzMet^TM
• Labeling for EM and LM with Silver-Enhanced Nanogold® and DAB
• Nanoprobes Wins New SBIR Grant
• Other Recent Publications
  • High-resolution Streptavidin-Nanogold® labeling of muscle fibers in Nemaline myopathy (NM)
  • Nano-W negative staining helps elucidate the mechanism of infection by Flock House virus (FHV)
  • Self-assembled monolayer (SAM) protected gold nanoparticle electrodes for sensitive voltammetric detection

• New Products: 5 nm Ni-NTA-Gold and Functionalized Gold Nanoparticles
  • 5 nm NTA-Ni(II)-Gold
  • Functionalized Gold Nanoparticles
• Get Close to Your Gold: Linker Lengths
• Charged Nanogold® Labels Endocytic Compartments in Yeast Cryosections
• NanoVan and the Genomic Analysis of Streptococcus pneumoniae Bacteriophages
• See us at Microscopy & Microanalysis 2009
• Other Recent Publications
  • Nanogold®-Silver and DAB double labeling 1: winners and losers among climbing fibers
  • Nanogold®-Silver and DAB double labeling 2: components of working memory
  • Optical manipulation of gold nanoparticles: propulsion with surface plasmon polaritons

• Double Labeling with Sequential Nanogold® and Gold Enhancement
• Monofunctionality: does it Always Work?
• HIV NEF Protein Targeting Revealed by Nanogold®
• EnzMet^TM: Best for ISH and IHC
• Nanoprobes Receives New Grant
• Other Recent Publications
  • FluoroNanogold used to elucidate the function of Yb protein in Drosophila
  • An immunoassay using resonance Rayleigh scattering detection
  • Plasmonic gold nanoparticles as photoaccoustic imaging contrast agents

• Nanogold® for Electron Cryotomography
• Best Approaches to Membrane Blotting and Lateral Flow Assays
• AuroVist^TM: Tips for Improved X-Ray Contrast
• Nanogold® and the Role of Syntaxin-18
• Reach the Right Department: Contact Information
• Other Recent Publications
  • Silver-enhanced Nanogold and enzymatic double labeling for EM
  • Bioconjugate imaging reagents for oncology applications in mouse models: review
  • A dendritic manganese-based contrast agent for MRI imaging

• Nanogold® with HQ Silver: Best Methods for Pre-Embedding EM Immunolabeling
• Nanogold® Reagents; Stability and Solubility
• Nano-W and 2-Dimensional Infrared Show Protein Amyloid Formation Pathway
• Nanogold® with Gold Enhancement, STED and CD44 Expression
• Guidelines for Custom Syntheses and Contract Research
• Other Recent Publications
  • More pre-embedding Nanogold® labeling with HQ Silver enhancement
  • Simple gold-based colony printing test for salmonella, no microscope needed
  • Gold nanoparticle-mediated amperometric silver deposition aflatoxin assay

• EnzMet^TM: Better for In Situ Hybridization, ideal for Microelectrode Array Biochips
  • EnzMet^TM for in situ hybridization (ISH) and biochips
  • EnzMet^TM for immunohistochemistry (IHC) and blotting
• Dispensing HQ Silver, and Controlling Nanogold® Stoichiometry
  • Solving a sticky situation: handling HQ Silver
  • Controlling stoichiometry: Nanogold® to biomolecule ratios
• NTA-Ni(II)-Nanogold®: New Technology for High-Resolution EM and Blots
• Nanogold® Labeling Helps Show Distribution and Function of SAP-1
• Nanoprobes wins Grants from NIH to Advance Imaging and Cancer Therapy
• Other Recent Publications
  • Mechanism of silver enhancement, using hydroquinone and silver nitrate
  • Electroanalytical determination of hydrogen peroxide using HRP-gold-zinc oxide-modified electrode
  • Hollow gold nanospheres (HGNs) as SERS labels for cancer markers in live cells

• Nanogold® and FluoroNanogold for SEM Labeling
• Nanogold® Enables Versatile Peptide Labeling
  • Label at cysteines with Monomaleimido Nanogold®
  • Label at lysines or N-terminal amines with Mono-Sulfo-NHS-Nanogold®
  • Label at C-terminal carboxyls with Monoamino Nanogold®
• Neuropeptide Y, Hippocampus-Dependent Seizures, and Nanogold®
• Nanogold® Helps Reveal Siliconization Mechanism
• Gold Nanoparticles, and When to Order from a Distributor
• Other Recent Publications
  • Gold nanoparticles as drug delivery vectors and therapeutic concentrators
  • Antibacterial activity of polymers incorporating gold nanoparticles and methylene blue
  • Gold-coated magnetite particles are more resistant and more easily functionalized

• FluoroNanogold: Tomography of the Cell Nucleus and Correlative EGFR Localization
• Alternative IgG Labeling Methods with Nanogold®
  • Hinge thiol labeling with Monomaleimido Nanogold®
  • Fc labeling using periodate oxidation and Monoamino Nanogold®
  • N-terminal or lysine residue amine labeling using Mono-Sulfo-NHS-Nanogold®
• GoldEnhance: Better for EM, Better for Blotting
• NanoVan Uncovers Possible Cause of Lafora Disease
• Beat the Price Increase - Order Before February 15
• Other Recent Publications
  • Silver nanoparticle dimers facilitate the study of SERS 'hot spots'
  • Secondary "slipping" interactions stabilize DNA-gold nanoparticle conjugates
  • Why gold nanoparticle-DNA conjugates resist enzymatic digestion

Volume 9: 2008

• Double Labeling for EM: Silver-Enhanced Nanogold® and DAB
• Nanogold® Labeling on Surfaces
• AuroVist: More Contrast for X-Ray Imaging
• Nanogold® and GoldEnhance Track Cell Migration
• Nanoprobes: New Year Hours - and Order Now at 2008 Prices
• Other Recent Publications
  • Gold Quantum Dots? Fluorescent Gold Nanoparticles.
  • Metal nanoparticles enhance Fluorescence resonance energy transfer (FRET).
  • Highly monodisperse 10 to 100 nm platinum nanoparticles.

• Charged Undecagold and Nanogold® Immunolabeling identify Bacillus subtilis Periplasm Components
• Nanogold® Labeling for Other Functional Groups
  • Hydroxyls
  • Aldehydes and Ketones
  • Carboxylic Acids
  • Phosphates in Lipids
• Tracking Down Food Poisoning with NanoVan
• Nanogold® Particles and In Vitro Budding of Intralumenal Vesicles
• Nanoprobes: Holiday Hours and Contacts
• Other Recent Publications
  • Silver-enhanced Nanogold® labeling shows Atg8 is essential for formation of autophagic isolation membranes.
  • Preparation of "nanobags" through controlled flocculation of gold or iron oxide nanoparticles.
  • Super-resolution nanoparticle tracking using live cell plasmon coupling microscopy.

• FcRn-Mediated Antibody Transport Revealed by Electron Tomography with Gold-Enhanced Nanogold®
• Monomaleimido Nanogold® Labeling: Reducing and Separating your Biomolecule
• Nanogold® Labeling for AFM
• Double Labeling for Electron Microscopy with DAB and Silver-Enhanced Nanogold®
• Want to Work with Us?
• Other Recent Publications
  • Gold-enhanced Nanogold® for backscatter detection SEM imaging.
  • Polymeric PARACEST agents for enhancing MRI contrast sensitivity.
  • Locally injected metallic gold for local anti-inflammatory therapy after brain trauma.

• NTA-Ni(II)-Nanogold®: Label with Submolecular Precision
• Nanogold® for Labeling Synthetic and Natural Oligonucleotides
  • Labeling of synthetic oligonucleotides
  • Labeling of plasmids and other naturally occurring oligonucleotides
  • RNA Labeling
• FluoroNanogold and GoldEnhance for Neuroscience
• Post-Embedding Immunoelectron Microscopic Labeling with Nanogold®
• New Grants Extend Medical Imaging Applications with Gold
• Other Recent Publications
  • Polymer-encapsulated nanoparticle dimers and trimers.
  • In vivo biotinylation for electron microscopic labeling.
  • Sonoelectrochemical preparation of gold nanoparticles.

• Charged Nanogold® Reveals Endocytic Pathways
• Customize your Silver enhancement
• Towards Correlative Double Labeling with FluoroNanogold
• Coffee, Calcium and the Ryanodine Receptor - Localized by Nanogold®
• Reach the Right Person: Contact Information
• Other Recent Publications
  • Peptide-mediated assembly of gold nanoparticles.
  • FluoroNanogold and GoldEnhance show how lipid droplets are arrested on the ER by lipidated apolipoprotein B-100.
  • MALDI imaging mass spectrometry for direct analysis of target distribution in tissues.

• Correlative and Dual Labeling with FluoroNanogold
  • Correlative fluorescence and transmission electron microscopic labeling
  • Checking labeling by fluorescence before processing for electron microscopy
  • Combined fluorescence and scanning electron microscopy
  • Correlative optical and X-ray fluorescence microscopy
• How to Gold-Label Peptides
• Gold Nanoparticles: Nanotechnology for Medical Imaging
• S-Layer Proteins, Lipid Bilayers, and Nano-W
• Ask for our New Catalog, and See Us at Microscopy and Microanalysis 2008
• Other Recent Publications
  • Enzymatic ligation of oligonucleotide-gold conjugates: amplified nanostructures.
  • Photovotaic preparation of large silver nanoprisms from silver nanoparticles.
  • Combined radiocopper - iron oxide nanoparticle probes for combined MRI and PET imaging.

• EnzMet for ISH, IHC, Blotting, EM...and More
  • In Situ Hybridization (ISH) and Immunohistochemistry (IHC)
  • Western and Protein Blotting
  • Electron Microscopy
  • Conductive Array Biochips
• Gold Enhancement: Better than Silver?
• The Secret of Extreme Radiotolerance, Exposed with Nano-W
• Nanogold® and Addiction
• Nanoprobes at Microscopy and Microanalysis 2008
• Other Recent Publications
  • Nanogold® with gold enhancement illuminates Golgi transport.
  • A rapid bio-barcode assay for low copy number virus detection.
  • Chiral inversion of thiol-stabilized gold nanoparticles by thiol exchange.

• Silver Enhancement of Nanogold® during Freeze Substitution
• Silver Enhancement: Choosing and Using - HQ or LI Silver
• Quantitating Heavy Metal Stains: STEM Tomography
• Nanogold®-Fab' and GoldEnhance for Precision Labeling
• DNA Nanodevices: See You in Jena
• Other Recent Publications
  • Nanogold® with silver enhancement and peroxidase-DAB: double labeling.
  • Preparation of platinum nanoparticles and effect on c. elegans lifetime.
  • Preparation and assembly of "cotton-like nanogold" fibers.

• In Vivo Labeling with Nanogold®
• Osmium and Silver Enhancement: Avoiding Etching
• Nanogold®-PEG Improves Delivery of Exon-Skipping Oligonucleotides for MD Therapy
• Nanogold®, DNA, or Proteins Organized by DNA Polycatenanes
• DNA Nanodevices: See You in Jena
• Other Recent Publications
  • Nanogold$#174; with silver and gold enhancement used to elucidate distinctive condensed ultrastructure of the facultative heterochromatin of the inactive X chromosome.
  • Nanogold$#174; and silver enhancement confirms that huntingtin decreases ubiquitin proteasome system activity in the synapses of Huntington's disease mice.
  • A method for isolating discrete 5 and 20 nm oligonucleotide-gold conjugates.

• Nanogold®-Fab' for CryoEM: Smaller is Better
• If You Need Colloidal Gold...
• Nanogold® with Silver Enhancement for Light Microscopy
• Gold and Other Nanotechnology Approaches to Cancer Therapy
• Distributor and Price Information
• Other Recent Publications
  • SERS Microscopy using antibody (Ab) conjugated to composite organicinorganic nanoparticles (COINs) as labels.
  • Spectral imaging for improved triple enzyme chromogenic labeling and staining.
  • Electron tomography: 4th International Conference papers, and Nanogold® and undecagold revisited.

• Sensitive Compact Disk-Based Immunoassays and Biochips with Silver-Enhanced Nanogold®
• What to Expect when Labeling Smaller Molecules with Nanogold®
• Nano-W Helps Characterize a Stable Cell Line for Generating Ebola Virus-like Particles
• Nanogold® with HQ Silver: Ideal for Pre-Embedding Labeling for Neuroscience
• New Catalog: Ask for Your Copy Now!
• Other Recent Publications
  • Inorganic nanoparticles as DNA transfection reagents: comprehensive review.
  • high relaxivity macromolecular contrast agents based on the conjugation of gadolinium chelates to MS2 viral capsids.
  • DNA linker-mediated crystallization of gold nanoparticles.

• Superior Protein Blotting with EnzMet
• Improved Labeling with Mono-Sulfo-NHS-Nanogold®
• Ni-NTA-Nanogold® and the Structure of the gamma-Tubulin Small Complex
• Towards Larger Gold Labeling
• New Guidelines for Custom Syntheses and Contract Research
• Other Recent Publications
  • Synthesis of DNA nanowires using 'click' reaction of azide-functionalized gold with alkyne-modified DNA.
  • Gold nanoparticles enhance the anti-leukemia action of a 6-mercaptopurine chemotherapeutic agent.
  • One-pot preparation of size-controlled bimagnetic Iron-Platinum-Iron Oxide heterodimer nanocrystals.

Volume 8: 2007

• Ni-NTA-Gold Technology: Nanogold for EM, GoldiBlot for Detection
• How to Get the Best out of Ni-NTA-Nanogold®
• Monoamino Nanogold®: Localization of Sugars in the Scrapie Prion Protein
• Pre-embedding Immunogold Labeling: Best Procedures with Nanogold®
• Nanoprobes Holiday Hours
• Other Recent Publications
  • Transfection of oligonucleotides with gold nanoparticles: loading determines uptake.
  • Advantages of gold nanoparticles in gene delivery systems based on high molecular weight chitosans.
  • Pre-embedding Nanogold localization of centrosomal proteins in primary cilia formation.

• EnzMet: A Quantum Leap for ISH and IHC Visualization
• GoldiBlot: Detect His-tagged Recombinant Proteins on Blots in One Hour
• More on AuroVist
• Charged Nanogold® Identifies Endocytic Pathways in Pollen Tubes
• Visit Nanoprobes at ASCB and See our New Catalog
• Other Recent Publications
  • Nanogold labeling and LI Silver enhancement in ultrathin cryosections.
  • Pre-embedding Nanogold localization of a G protein-coupled receptor for estrogens.
  • Tunable control of refractive index in gold nanoparticle solutions.

• 3-D Distribution of Nanogold® and Undecagold by STEM Tomography
• Improved Protocols for Blots
• GoldEnhance Enables HPF/FSF EM of Endocytosed Nanogold®
• Nanogold® Achieves Ultrastructural localization of Fras1
• Look for Nanoprobes at ASCB
• Other Recent Publications
  • Surface-Enhanced Raman Spectroscopy (SERS) for detection of DNA on silver nanoparticles.
  • Pre-embedding Nanogold and post-embedding 15 nm gold: cellular and tissue localization of globotriaosylceramide in Fabry disease.
  • Folic acid and PEG-derivatized gold nanoparticles for cellular uptake and cancer targeting.

• Nanogold® Used for First Description of SNARE Proteins on Extracellular Surface
• Calculating Labeling with Nanogold® and with NTA-Ni(II)-Nanogold®
• Sequential Larger Gold and Fluorescence Labeling
• Multiple Correlative Immunoelectron Microscopy and Immunofluorescence
• Updated Material Safety Data Sheets
• Other Recent Publications
  • Better preservation and retention of DNA.
  • Gel electrophoresis separation of nanoparticles by composition and shape.
  • Ultrasensitive label-free binding studies using back-scattering interferometry.

• Combined Fluorescent and Larger Gold Labeling
• Labeling Fc or Hinge IgG Sites with Nanogold®
• Undecagold with Cryoelectron Microscopy: Probing Myosin Interactions
• NTA-Ni(II)-Nanogold® Used to Solve Cryo-EM Structure of TOR-KOG1
• Enzyme Metallography, and Other News
• Other Recent Publications
  • The structure of PilG, as determined with NTA-Ni(II)-Nanogold® and EM.
  • NanoVan with 2-D crystallography and 3-D reconstruction yields protein kinase C delta structure.
  • Multiplexed biomarker detection and cell identification with gold nanorods.

• Enzyme Metallography: New Paper and Coming Products
• Nanogold Labeling: Filtration, Purification, and Beating Aggregation
• NTA-Ni(II)-Nanogold® Reveals Lipoprotein-Secretin Interactions
• Nanogold®, Synaptic Vesicle Membrane Retrieval and Synapse Development
• The Right Contacts: Ordering, Technical Questions, and Custom Synthesis
• Other Recent Publications
  • FluoroNanogold: check labeling by fluorescence before quantitative gold labeling analysis.
  • Metal enhanced fluorescence: effect of metal particle coupling.
  • Structure determined by Nanogold labeling used as template for design of FRET study.

• FluoroNanogold: One Probe Gives You Fluorescence and Gold Labeling
• Eliminating Background staining with Nanogold® and Silver Enhancement
• HQ Silver Gives Better Results with All Types of Gold
• Undecagold: The Smallest Commercial Gold Label
• Nanoprobes at Microscopy & Microanalysis 2007
• Other Recent Publications
  • Gold nanocages enhance photothermal therapy.
  • Multiple immunohistochemical staining with antibodies from the same species.
  • Five-color fluorescence imaging of separate lymphatic basins.

• Ultrastructure of Lipoproteins with NTA-Ni(II)-Nanogold®
• Multivalent Probes: Conjugating Multiple Biomolecules to one Nanogold®
• AuroVist: The First Gold Nanoparticle X-Ray Contrast Agent
• Colloidal Gold and Nanogold® in Immunochromatographic Assay Devices
• Look for us at the Long Island Life Sciences Summit
• Other Recent Publications
  • HQ Silver enhances ultrasmall gold
  • Albumin-plasmid nanoparticles as corneal intraceptor expressing agents.
  • Fluorescence microscopy at the nanoscale.

• GoldEnhance and Nanogold® Double Labeling
• Nanogold® Labeling Reagents: Avoiding Aggregation
• Nano-W Helps Improve Antiviral Targeting
• Enhanced Plasmid DNA Transfection with Positively Charged Nanogold®
• Going to the Venture Forum? See You There!
• Other Recent Publications
  • Antibacterial properties of gold-conjugated drugs: a new drug delivery system?
  • An immunoresonance scattering spectral assay for trace prealbumin.
  • Nanogold® pre-embedding immunolabeling helps demonstrate role of Cadherin-8 in coupling cold-sensitive sensory neurons and their target DH neurons.

• Announcing AuroVist: The First Gold Nanoparticle X-Ray Contrast Agent
• Using AuroVist: Tips for Success
• What does Retinoschisin do? Find Out with Nanogold®
• Nano-W Helps Screen Inhibitors of Amyloid Beta Toxicity
• Nanoprobes at First Australian Correlative Microscopy Workshop
• Other Recent Publications
  • Gold nanorods as probes for mechanical tension.
  • A high quantum yield gold nanoparticle near infrared luminescence probe.
  • Core-shell silver nanoparticles for metal enhanced fluorescence and single nanoparticle sensing.

• Nanogold® and Quantum Dot Molecular Beacons
• Make Combined Fluorescent and Gold Labeling Work for You
• Nanogold® and the Link Between Medial Prefrontal Cortex and Hippocampus
• Nanogold® and HQ Silver: Ideal for Pre-Embedding Labeling
• Where to Find Technical Help
• Other Recent Publications
  • Pre-embedding Nanogold combined with post-embedding colloidal gold labeling of Tudor proteins.
  • Gold nanoparticles enhance biohydrogen generation from artificial wastewater.
  • Gold nanoparticle biosensors for simple, ultrasensitive detection of protein-lectin interactions.

• NTA-Ni(II)-Nanogold®: Smaller Probe, Higher Resolution
• Get the Best out of NTA-Ni(II)-Nanogold: Tips and Tricks
• GFP and Nanogold for Correlative Fluorescent and Gold Labeling
• Electron Tomography with Nanogold®
• Nanoprobes Wins Phase 2 SBIR Grant on Gold MicroCT Imaging Agents
• Other Recent Publications
  • Nano-W helps in the development of inhibitors for Alzheimer's Disease.
  • Gold nanoparticles stabilize platinum platinum (Pt) oxygen-reduction fuel-cell electrocatalysts.
  • Divalent gold nanoparticles with specific directional functionalization.

Volume 7: 2006

• Nanogold® in Neuroscience: Spinal Cord Interneurons and Potassium Channels
• Troubleshooting: Nanogold® Conjugates
• FluoroNanogold: Check your Labeling before EM Processing
• GoldEnhance: Better Enhancement for Nanogold® and Colloidal Gold
• Nanoprobes Holiday Hours
• Other Recent Publications
  • Combined Nanogold® and green fluorescent protein (GFP) labeling.
  • Hybrid gold-silica-quantum dot structures: interactions of gold particles with light.
  • Nanoparticle-polymer composites: preparation and applications.

• Deconstructing Photosystem II with NTA-Ni(II)-Nanogold®, Continued...
• Nanogold® Labeling Reagents: Better Performance and Updated Instructions
• NanoVan and Nano-W Help Resolve a Sticky Situation
• Nanogold® Shows How Skeletal Muscle Burns Fat
• Changes to Newsletter Sign-up and Security
• Other Recent Publications
  • Rigid, self-assembling triangular DNA motifs for periodic ordering of 5 and 10 nm gold particles in two dimensions.
  • Building blocks for nanotechnology: gold nanoparticles functionalized with polypyridyl ruthenium complexes.
  • Single-walled carbon nanotubes labeled with quantum dots or gold nanoparticles: luminescence quenching interactions.

• Nanogold® as a Marker for Viral Drug Delivery
• How Well did my Reaction Work: Calculating Labeling
• GoldEnhance Visualizes Gold Nanoparticle Cancer Targeting
• Nanogold® Penetrates the Nucleus to Find HuD
• Ordering and Shipping Deadlines
• Other Recent Publications
  • HQ Silver used with ultrasmall colloidal gold to study matrix metalloproteinase in cancer progression.
  • Autoradiographic labeling combined with immunohistochemistry and multiple immunofluorescence.
  • Autoradiography and post-embedding immunogold used to study role of megalin in Sonic hedgehog trafficking.

• Enzyme Metallography for Light and Electron Microscopy
• Polyfunctional Nanogold®
• Nanogold® and Lyosomal Storage Disease
• Nanogold® and Undecagold Beams for Matter Wave Interferometry
• Other Recent Publications
  • Gold Enhancement of colloidal gold used to investigate Collagen XII interactions.
  • Autometallographic methods for the detection of metal deposits, nanoparticles, and quantum dots: a review.
  • Silver-enhanced gold biochips for visual and optical detection of pathogenic bacteria.

• A Nanogold®-Quenched RNA Molecular Beacon
• Storing Nanogold® Conjugates
• FluoroNanogold: Correlative Fluorescence Microscopy and FESEM
• Nanogold® Characterization of MYPT2, a Target Subunit of Myosin Phosphatase
• Custom Syntheses and Distributor Pricing
• Other Recent Publications
  • Ultrasensitive detection: amplification of biorecognition events through the catalytic evolution of a biocatalytic label.
  • Multiple electron microscopic labeling by particle shape, size and composition.
  • Reliable and reproducible digital immunogold analysis for electron microscopy.

• Correlative XRF and Immunofluorescence with FluoroNanogold
• FluoroNanogold In Situ Immunolabeling for Placental Protein Localization
• Gold Labeling of RNA and Oligonucleotides...Revisited
• Precision Nanogold® Labeling Reveals Role of Sfi 1p Centrin Filaments in Yeast
• Ordering and Custom Synthesis: Some Reminders
• Other Recent Publications
  • Nanogold demonstrates role of Schwann cell differentiation in carcinomas of the salivary glands.
  • DNA-induced separation of gold nanoparticle-DNA conjugates based on nanoparticle size and melting temperature.
  • Review of novel FRET fluorophores, quenchers and methods for bioconjugation.

• Localization and Role of HLA-G: ReAsH, Nanogold® and GoldEnhance
• Labeling Aromatic Groups
• Pre-embedding Nanogold® Labeling Localizes Cannabinoid Receptors
• Post-Embedding Nanogold® Labeling of Siliceous Spicules in Desmosponges
• Nanoprobes at Microscopy and Microanalysis 2006
• Other Recent Publications
  • Highly sensitive nanoparticle-based bio-barcoding assay.
  • Hybrid Platinum-Cobalt / Gold particles with tunable domains, and amphiphilic gold-iron oxide "Janus" particles.
  • Catalytic signal amplification by metal ion release and fluorogenic substrate conversion.

• Correlative Fluorescence and SEM with FluoroNanogold
• How to Obtain the Cleanest Gold Enhancement
• Nanogold® Labels Gene Therapy Vector
• Inhibition of Insulin Fibrillogenesis: Using Nano-W to Study Aggregation
• Price Adjustment: Order by May 31 to Take Advantage of Current Prices
• Other Recent Publications
  • Nanogold® pre-embedding electron microscopic localization of stargazins in rat brain.
  • Fluorescence lifetime quenching near 1.5 nm gold.
  • Effect of size and shape on intracellular uptake of gold nanoparticles.

• Nanogold® Identifies Neuron Development Promoter
• Osmium and Silver: How to Avoid Etching
• Nanogold® with LI Silver for CryoEM and Gel Staining
• Nanogold® Helps Elucidate Endocytic and Secretory Trafficking in Arabidopsis
• Nanoprobes Address Change
• Other Recent Publications
  • Metal nanoparticles enhance fluorescence.
  • Stable spherical gold particle assemblies.
  • Femtomolar electrochemical detection using metal sulfide nanoparticles.

• Gold Nanoparticles as X-Ray Contrast Agents
• Background Troubleshooter: Different Probes and Enhancement Methods
• NTA-Ni(II)-Nanogold®: Role of Wzc in the Assembly of e. coli Group 1 Capsules
• Colorimetric Kinase Activity Detection by Nanoparticle Modification
• Nanoprobes, Incorporated Completes Major Expansion
• Other Recent Publications
  • Toxicity of gold nanoparticles: a surface property?
  • Distance dependence of fluorescence quenching by 13 nm gold.
  • Effectof hydration upon UT-A and UT-B in rat kidney: Nanogold® pre-embedding immunoEM.

• Nanobiotechnology: DNA Nanogrids as Templates for Gold Particle Assembly
• "Help, it's Stuck on the Column!" Rescue Bad Nanogold® Separations
• Enzyme Metallography for Ultrasensitive Electrical Detection on DNA Chips
• Nanogold® Labeling for ICP-Mass Spectrometry Assays
• Other Recent Publications
  • Nano-W negative staining of viruses, redux.
  • Preparation of large cobalt nanoparticles.
  • Silver-gold nanowires prepared in detergent thin films.

• Nanogold® Labeling Maps Siliconization in Sponges
• Nanogold® Labeling of Plasmids
• High Resolution Negative staining with NanoVan and Nano-W
• Inosine Monophosphate Dehydrogenase, Functionally Analyzed with Nanogold®
• New Telephone Extensions
• Other Recent Publications
  • DNA tiles as array templates for gold nanoparticles.
  • Multi-wall silver nanotubes.
  • Delivery of water-soluble gold-TAT peptide conjugates to the cell nucleus.

Volume 6: 2005

• Enzyme Metallography for Precise Immunohistochemistry
• Alternatives for Antibody Labeling
• NTA-Ni(II)-Nanogold® Reveals Multienzyme Complex Structure
• Undecagold for Cryo-EM - with Image Analysis
• Other Recent Publications
  • Silver-dendrimer nanocomposites as cell biomarkers.
  • Conductive polymer-encapsulated gold nanoparticles as biosensors.
  • A clonable gold label: metallothionein.

• Validation of a Chromogenic Dual ISH and IHC Assay for HER2 Gene and Protein Status
• Labeling other Functional Groups: Phosphates in Lipids
• Double and Triple Labeling with Silver-Enhanced Nanogold® and HRP
• Keeping GoldEnhance Under Control
• MSDSs Now Available Online
• Other Recent Publications
  • Nano-W helps solve structure of lobster clottable protein.
  • Pre-embedding Nanogold® labeling reveals expression of a novel lectin.
  • Nanogold anti-digoxigenin for mRNA in situ hybridization.

• NTA-Ni(II) Gold Finds the Photosystem II PsbH Subunit in Synechocystis 6803
• Bigger NTA-Ni(II) Gold
• Nanogold Labeling: pH and Solvent
• SEM-ISH: Bacterial Detection by rRNA Sequence using Gold-Enhanced Nanogold®
• Who You Gonna Call? Contact Information
• Other Recent Publications
  • Pre-embedding Nanogold® labeling establishes distribution and role of rootletin.
  • Control of colloidal gold aggregation by citrate ion replacement.
  • Controlled coalescence of monolayer-protected gold nanoparticles by radical thiol abstraction.

• Tetrairidium Carbonyl as a Microspectroscopy Probe
• What is the Difference between HQ and LI Silver?
• Towards Bigger Nanogold®
• In Vivo Vascular Casting with Gold Nanoparticles
• Other Recent Publications
  • One-day FISH procedure.
  • Standardized protein-embedding technique for immunohistochemistry.
  • Diamine Oxidase-gold for histochemical staining of histamine in basophils and Mast cells.

• Nanogold® as an AFM Label
• Best Ways to Separate Nanogold® Conjugates
• Correlative Double Immunoelectron Microscopy and Double Immunofluorescence
• Triple EM Immunolabeling: Gold, Palladium and Platinum
• Nanoprobes Adds Space and Staff
• Other Recent Publications
  • Quadruple immunoelectron microscopic labeling by particle shape.
  • New methods for evaluating fluorophore colocalization by image analysis.
  • A DNA-gold nanowire-based SQUID.

• Double Labeling: Nanogold®, 10 nm Gold, and Gold Enhancement
• Labeling Other Functional Groups: Carboxylic Acids
• Biocatalytic Growth of Shaped Gold Nanoparticles
• Nanogold® Labeling Demonstrates Adenoviral Targeting Modification
• Other Recent Publications
  • Luminescence of gold nanoparticles.
  • A simple gold nanoparticle/polyaniline memory device.
  • Stepwise assembly of nanoparticle layers by coordination chemistry.

• Nanogold®-Streptavidin As a Tertiary Probe for Pre-embedding Labeling
• Nanogold Labeling: Aggregation, Antibodies and Oligonucleotides
• Nanogold-Fab' Labeling in Platelets: Regulation of Granule Secretion
• Nanowires from Gold Enhanced Nanogold
• Price Quotations
• Other Recent Publications
  • NTA-Ni(II) Nanogold with negative staining for protein ultrastructure.
  • Gold nanoparticle SPR cancer sensors for use in living cells.
  • Once-dimensional gold nanoparticle arrays via rolling circle amplification template generation.

• NTA-Ni(II)-Nanogold® Identifies His Tag Locations on Viral Capsids
• Fluorescence Quenching, FluoroNanogold and Molecular Beacons
• Nanogold® Optical Sensors for Chemical Warfare Agents and Pesticides
• How to Label Aromatic Amines
• See you in Hawaii!
• Other Recent Publications
  • A colorimetric nerve gas biosensor through inhibition of enzymatic gold nanoparticle growth.
  • NanoVan helps reveal architecture of the bacteriophage T4 primosome.
  • Permanent non-aqueous mounting of immunofluorescently stained specimens.

• FluoroNanogold: Check Your Labeling before Electron Microscopy
• Labeling Other Functional Groups: Aldehydes and Ketones
• Nanogold® Cryo-EM Yields Structure of Enhancer-Binding Protein
• Nanogold® is for SEM Labeling, too!
• Other Recent Publications
  • Gold nanocages as optical contrast agents.
  • Nano-W helps determine role of protein in viral life cycle.
  • Colloidal gold labeling and shelf life.

• Cryo-EM: Nanogold® Hits the Spot
• Labeling Other Functional Groups: Hydroxyls
• NanoVan for Viral Visualization
• Protein Array with Silver-Enhanced Gold Detects Myocardial Infarction
• Other Recent Publications
  • Pre-embedding silver-gold and peroxidase double labeling.
  • Heat shock protein arrays as templates for metal nanoparticles.
  • Raman spectroscopy for surface plasmon studies.

• Enzyme Metallography for In Situ Hybridization
• Immuno-EM with Tyramide Signal Amplification and Nanogold® Detection
• Dividing Nanogold® for Multiple Reactions
• More on the Preparation and Activity of Gold Nanoparticles
• Nanoprobes at USCAP
• Other Recent Publications
  • Autometallograpy of zinc - review.
  • Platinum nanoparticles from hydrosilylation reaction.
  • Tunable silver nanoparticle SERS substrates.

• Nanowires from Nanogold®
• Just What is Nanogold®, Anyway?
• Gold Surfaces Change Reduction Potentials: Catalysis
• Nanogold®-Silver and Enzyme Double Labels Neuronal Connections
• Other Recent Publications
  • Gold nanoparticle array detection.
  • Gold-coated iron oxide nanoparticles.
  • Universal antigen retrieval with citraconic anhydride.

Volume 5: 2004

• Gold Lipids and GoldEnhance: Presenting...Antigens!
• More Tips for Labeling Site Selection and Conjugate Separation
• Combined Undecagold and Fluorescently Labeled Oligonucleotides
• Nanogold® Labeling of Plant Tissues
• Custom Synthesis at Nanoprobes
• Other Recent Publications
  • Silver-enhanced Nanogold and ABC-DAB double immunohistochemistry.
  • Triggered amplification by hybridization chain reaction (HCR).
  • Immersion autometallography for zinc.

• Concentration of Nanogold®, and How to Measure
• Negative Staining: NanoVan in Electron Microscopy of Microsporida
• Receptor Recycling Elucidated with Nanogold® Double Labeling
• Gold Lipids: Decorated Layers and Liposomes
• Nanoprobes at the San Antonio Breast Cancer Symposium
• Other Recent Publications
  • Copper (II) quenchers for molecular beacons.
  • Signal amplification with self-ligating DNA and RNA probes.
  • Preparation of silver nanocubes.

• Nanogold® Enhances Radiation Therapy of Cancer
• Nanogold®: Charge and Thermal Sensitivity
• Localizing Nuclear Scaffold Components with Nanogold®
• Gold-Conjugated DNAzymes for Colorimetric Lead Detection
• Nanoprobes ISH Technology at the Brisbane IAP Meeting
• Other Recent Publications
  • Infrared quantum dots.
  • Peptide-based photosensitizing molecular beacon.
  • Microwave irradiation improves labeling of bone.

• Nanogold®: Plan your Labeling for Best Results
• Enzyme Metallography: a New Stain for Light and Electron Microscopy
• Nanogold® Elucidates Targeting of Hepatitus C Core Protein
• Gold-Based Fluorescence and Colorimetric DNA Detection
• Other Recent Publications
  • Gold-DNA-Quantum dot constructs.
  • HQ Silver enhanced gold for RNA in situ hybridization.
  • Ultrasensitive biosensors: enzymes enhanced by gold surfaces.

• FluoroNanogold: Best Strategies for Combined Fluorescent and Gold Labeling
• Nanogold® Used to Study Neuronal Glutamate Receptor Activation
• Engineered Viruses for Nanopatterning Gold Particles
• Laser-Activated Capsules of Silver Nanoparticles
• Other Recent Publications
  • Quantitation of gold labeling.
  • HQ Silver enhancement of small gold.
  • Whole mount RNA in situ hybridization with silver-enhanced gold.

• Novel Reactivities and Polyfunctional Nanogold®
• Double Immunogold Labeling with Nanogold® and Colloidal Gold
• Getting the Best out of NTA-Ni(II)-Nanogold®
• Silver-Enhanced Nanogold® and Immunoperoxidase Double Labeling
• Other Recent Publications
  • HQ Silver enhances colloidal gold, too.
  • DNA interacts with cationic gold nanoparticles.
  • Pre-embedding Nanogold labeling helps link calcium overload with apoptosis.

• Increase Labeling Resolution with Nanogold®: Label Smaller Molecules
• Double Labeling Using Differential Gold Enhancement with Nanogold®
• Solubility Properties of Nanogold®: How Best to Dissolve and Label
• Gold particles for Surface Plasmon Resonance Detection
• Nanoprobes at Microscopy & Microanalysis '04
• Other Recent Publications
  • Enhanced SPR sensor using nanoholes in gold film.
  • High-aspect gold nanorods.
  • A new method for preparing stable, water-soluble platinum nanoparticles.

• Online Ordering Available Again
• Nanogold®-Labeled Oligonucleotides: Conjugation and Separation
• FluoroNanogold: New Probes for Correlative Labeling
• Double Labeling with DAB and Silver-Enhanced Nanogold®
• Some Advantages of Gold Enhancement with Nanogold®
• Other Recent Publications
  • Electrophoretic behavior of oligonucleotide-gold.
  • A new method for preparing protected silver clusters.
  • Increasing the sensitivity of postembedding immunogold.

• Welcome to the New, Leaner Newsletter!
• Nanobiotechnology: Self-Assembled Metallated DNA Nanotubes
• Nanogold® Labels Nuclear Features: Function of NO66 Protein
• Negative Staining Helps Visualize Immunogold
• Gold Nanoparticles for Protein Immobilization and AFM Studies
• Other Recent Publications
  • Pre-embedding Nanogold in Neuroscience.
  • Silver-enhanced gold for DNA hybridization arrays.
  • Autometalographic and histofluorescent chelatable zinc localization.

• What do you look for in Immunogold: Help us make it better
• Advantages and Uses of Gold-Labeled Peptides
• How Big is Nanogold®? What is the Concentration of Conjugates?
• Gold Enhancement for SEM Immunogold in Metallic Environments
• Gold Nanoparticles as Drug Carriers
• Other Recent Publications
  • Silver and gold enhanced Nanogold-protein nanowires.
  • Engineering amyloidicity in nanofibrillar materials.
  • Fluorescent TRAP staining.

• Gold-Labeled Liposomes Reveal HDL Cholesterol Trafficking
• Nanoprobes at USCAP: Brightfield ISH Staining for HER2 Amplification
• Silver-Enhanced Undecagold Probes Show Mitochondrial DNA Uptake
• Nanogold® Labeling for MALDI Mass Spectrometry, PAGE and SEM
• Useful Pre-Embedding Nanogold® Labeling and Gold Toning Protocol
• Other Recent Publications
  
  • 50 immunostains at once.
  • Silver carbonate mitochondrial staining.
  • Pre-embedding Nanogold® and post-embedding colloidal gold.

• Nanogold® Resolves the Distribution of PECAM-1
• Fluoronanogold shows Path of Antisense Oligonucleotides to Targets
• Combining Gold and Peroxidase Labeling for LM and EM in Neuroscience
• Nanogold® Labeling shows Relation of Proteins in Developing Rat Lens
• Other Recent Publications
  
  • Silver-enhanced gold sequential double labeling method.
  • Preservation of ultrastructure in c. elegans.
  • Catalytic dendrimer-nanoparticle conjugates.

Top of page

Volume 4: 2003

• Correlative Video LM/EM with Nanogold® and Goldenhance
• Silver Enhanced Nanogold® for Scanning Electron Microscopy
• DNA-Gold, Colloidal Gold, and the Role of Citrate
• Gold Nanoparticle Sensor for DNA Hybridization
• Nanoprobes at the San Antonio Breast Cancer Symposium
• Other Recent Publications
  
  • Fluorobodies.
  • Colloidal crystals with tunable colors as photonic papers.
  • One-step dual staining method for Mast cells.

• FluoroNanogold Shows 3D Organization of Pki-67
• Nanogold® Labeling: Multiple Conjugation, and Ionization
• NanoVan Negative Staining Reveals Prion Architecture
• Nanogold® and the Chloroplast Outer Envelope Translocon
• Nanoprobes at the Association for Molecular Pathology Meeting
• Other Recent Publications
  
  • Hyper-Rayleigh scattering immunogold assay.
  • Synthesis of stable metal nanoparticles in microgels.
  • Optical imaging.

• Silver Nanowires Templated by DNA
• Nanogold® Reveals Function of Eukaryotic RNA Helicase
• Gold Nanoparticles in Cell Imaging and Cancer Therapy
• The Further Adventures of Nanogold® in Neuroscience
• Other Recent Publications
  
  • Preparing plant tissues for FISH.
  • Fabrication of novel biomaterials through molecular self-assembly.
  • Controlling anisotropic nanoparticle growth; bio- bar codes.

• Introducing: Ni-NTA-Nanogold®
• A Combined Nanogold® and Texas Red Neuronal Tracer
• HQ Silver Enhances Nanogold® and Colloidal Gold
• Controlling the Interaction of DNA with Gold Nanoparticles
• Complete Price List Online
  
• Other Recent Publications
  
  • Surface-enhanced infrared spectroscopy as a biodetection method.
  • Optical model of conjugates of colloidal gold.
  • 2 nm gold glycoconjugate.

• New Nanogold® Pre-Embedding Labeling Procedure
• Building Blocks for Nanotechnology: Chaperoned Nanogold®
• Nanogold® Labeling for Correlative Fluorescence and SEM
• Gold Nanoparticle Biochips and Sensors
• Other Recent Publications
  
  • Cowpea mosaic virus as a nanotech building block.
  • 3-D immunogold by HAADF-STEM.
  • Antigen retrieval for postembedding EM.

• Nanogold® Helps Demonstrate Hierarchical Folding in Chromosomes
• Nanovan and Nano-W: 'Tunable' Negative Staining
• Nanogold® and Colloidal Gold, Pre- and Post-Embedding Immuno-EM
• Nanoprobes at Microscopy & Microanalysis '03
• Other Recent Publications
  
  • Fixation for immunogold labeling of GFP.
  • Novel combined fluorescent and gold probe.

• Alexa Fluor Fluoronanogold: New Papers Demonstrate Advantages
• Optimizing Staining with Gold Enhancement
• Applications of Nanogold®-Fab' Conjugates in Neuroscience
• Smaller Immunoreactive Gold Conjugates
• Other Recent Publications
  • TUNEL with pre-embedding immunoEM for detecting DNA strand breaks in apoptosis
  • Colloidal gold and enzymatic double labeling modification
  • DNA aptamers.

• Gold Cluster Labels in Cryo-EM: Structure of Filamentous Phage 4
• Finding Solutions to some Immunogold Labeling Problems
• Nanogold® Labeling of Bak: Role in Mitochondrial Permeability
• Double Labeling: Combining Silver-Enhanced Gold with other Labels
• Other Recent Publications
  • Correlative light and energy-filtered EM.
  • Estimating immunogold density.
  • Gold nanoparticle patterning.

• Nanogold® used for Nanowiring a Redox Enzyme
• New Applications of Gold Enhancement
• Conductive Polymers: Non-Charging EM Substrates
• Easier Browsing of Back Issues, and other News
• Other Recent Publications
  • Effect of fixation upon beta-galactosidase
  • Nanogold localization of Plakophilin 3.

• Gold Clusters - Nanotechnology Applications
• Nanogold® helps Identify Novel Factor in Golgi and ER Assembly
• Quicker, Accurate Technical Help and Custom Synthesis Questions
• New at Nanoprobes; USCAP Meeting Presentation
• Other Recent Publications
  • Fixation and gold labeling with GFP.