Because staining with EnzMet™ is similar to using conventional enzyme chromogens such as DAB, moving from DAB to EnzMet™ is usually a very easy transition.
Transitioning from other enzymatic methods is easy.
With the exception of the replacement of the DAB with the EnzMet™ development reagents, a typical EnzMet™ protocol uses the same reagents and procedure as the DAB protocol.
However, that does not mean nothing can go wrong, so here are a few guidelines and modifications that will help if you have questions or encounter problems:
EnzMet™ is frequently more sensitive than the corresponding DAB detection reagent.
As a result you can increase the dilution of the primary antibody.
In a study of a range of antigens, we find that 5-fold or 10-fold extra dilution is about right for many markers; however, depending on the target and its distribution, additional dilutions may vary between nothing and 50-fold.
Start with a 5-fold additional dilution and adjust up or down as necessary.
