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Buy now0.8 nm Undecagold Labeling Reagents


Ultra-small --The best choice for Cryo-EM.

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Undecagold (Au11) is smaller than Nanogold®, with a core of 11 gold atoms only 0.8 nm in diameter. It is ideal for ultra-high-resolution EM work such as cryo-EM, scanning transmission electron microscopy (STEM), or for resolving elements of large structures by TEM in conjunction with image processing. Undecagold has been used to see the biotin binding sites on avidin to 1 nm resolution by electron microscopy. It is prepared in a form with one reactive arm for cross-linking to a specific site on a target molecule, and is available with different reactivities for labeling different sites.

  • Ultra small 0.8 nm gold core.
  • Use to prepare the smallest possible gold probes.
  • Highest possible resolution.
  • Site specific covalent labeling with choice of reactivities.

Note: Undecagold is perfect for labeling in high-resolution applications like cryo-EM, but single Undecagold clusters are not routinely visualized directly in the TEM. For standard TEM, Undecagold may be seen upon image processing of protein helices and crystals, or visualized en masse if there is a bulk deposition such as staining of an organelle. Also, Undecagold develops more slowly and with less final developer deposition during silver enhancement or gold enhancement than with our larger, 1.4 nm Nanogold®. Therefore, for many applications we recommend 1.4 nm Nanogold®.

Undecagold Structure (27k)
Structure of undecagold showing the [Au11] core, coordinated ligands and peripheral reactive group (maleimide).


Buy nowApplications

 

Undecagold (Au11) Labeling Reagents

Buy nowMonomaleimido-Undecagold

The simple-to-use activated Maleimido-undecagold reagent is used for labeling thiols with undecagold. It will specifically label free sulfhydryls in proteins, antibodies, peptides, substrates or other molecules containing a free thiol. For use, it is simply dissolved in water and mixed with the material to be labeled for a few hours. Undecagold conjugates are then isolated by gel filtration.

Applications:

  • Create your own small gold probes by linking Au11 to your primary antibody Fab' fragments.
  • Label proteins directly for high resolution, site specific identification.
  • Label peptides containing cysteine.

 


Buy nowMono-Sulfo-NHS-Undecagold

This reagent is used to covalently attach undecagold to primary amines.

Applications:

  • Label proteins which do not contain a cysteine.
  • Label peptides at the N-terminal.
  • Undecagold-label small molecules as inhibitors or substrate analogs for probing transition state structures.

 


Buy nowMonoamino Undecagold

Use this reagent to label carbohydrates and glycoproteins, or activate with a heterobifunctional cross-linking reagent for different reactivity.

 


Buy nowCharged Undecagold

Positively and negatively charged undecagold may be used as ionic labels, to stain biological structures with opposite charges. Positive undecagold has multiple amines on its surface and may therefore stain negatively charged features; negatively charged undecagold has multiple carboxyl groups, and will stain features containing many positively ionizing groups.

 


Buy nowNon-Functionalized Undecagold

Non-fuctionalized undecagold is an inert, stable form of the undecagold particle with no reactive chemical functionalities or specific affinity properties.

Applications:

  • As a size standard for calibration in high-resolution electron microscopy.
  • Sizing pores (see: Schwartz, M. P., and Matouschek, A.: The dimensions of the protein import channels in the outer and inner mitochondrial membranes. Proc. Natl. Acad. Sci. USA, 96, 13086-13090 (1999)). [Complete references]
  • For tracing contiguous interior spaces in cells and tissues.

 


Custom Undecagold Conjugates

Nanoprobes does not stock undecagold conjugates, but we will be glad to prepare them to order as a custom synthesis. Let us know your needs, and we'll give you a quote: Use our online form, e-mail us at [email protected], or call us at 877-447-6266 (US toll-free number) or (631) 205-9490. We look forward to working with you!

 

Price List:
Undecagold Reagents
Custom undecagold conjugates are also available.
Paying by Purchase Order? Please use our Online Order Form.
Monomaleimido Undecagold
#2030
Monomaleimido Undecagold
Au11 cluster with single maleimide group for selectively labeling thiols (-SH). May be used to label primary Fab' antibody fragments, cysteine groups on proteins, or other sulfhydryl containing compounds. Reagent and procedure to label 300 mg Fab'. 50 nmol
$449
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#2030A
Monomaleimido Undecagold
in 5 aliquots

Same total quantity as 2030, but provided in 5 separate tubes. Since the malieide moiety is unstable once dissolved, separate aliquots are preferable if several smaller-quantity labeling reactions are planned at different times.

50 nmol
in 5 aliquots
$501

Qty:

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#2030S
Monomaleimido Undecagold introductory size
Same as 2030, but packaged in an introductory size for smaller labeling experiments. 10 nmol
$128
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Monoamino Undecagold
#2031
Monoamino Undecagold
Au11 cluster with a single primary amine. May be used for labeling the carbohydrate moiety of glycoproteins or other uses. 50 nmol
$308
Qty:

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#2031A
Monoamino Undecagold
in 5 aliquots

Same total quantity as 2031, but provided in 5 separate tubes. Preferable if several smaller-quantity labeling reactions are planned at different times.
50 nmol
in 5 aliquots
$355
Qty:

Code (Optional)

#2031S
Monoamino Undecagold introductory size
Same as 2031, but packaged in an introductory size for smaller labeling experiments. 10 nmol
$89
Qty:

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Mono-Sulfo-NHS-Undecagold
#2045
Mono-Sulfo-NHS-Undecagold
Undecagold with a single N-hydroxy-sulfosuccinimidyl group. Used for labeling primary amines. 50 nmol
$449
Qty:

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#2045A
Mono-Sulfo- NHS-Undecagold
in 5 aliquots

Same total quantity as 2045, but provided in 5 separate tubes. Preferable if several smaller-quantity labeling reactions are planned at different times.
50 nmol
in 5 aliquots
$501
Qty:

Code (Optional)

#2045S
Mono-Sulfo-NHS-Undecagold introductory size
Same as 2045, but packaged in an introductory size for smaller labeling experiments. 10 nmol
$128
Qty:

Code (Optional)

Charged Undecagold
#2043
Positively Charged Undecagold
Undecagold with a net positive charge; contains amino groups. 50 nmol
$309
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#2044
Negatively Charged Undecagold
Undecagold with a net negative charge; contains carboxyl groups. 50 nmol
$309
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Non-Functionalized Undecagold
#2060
Undecagold Particles, Non- Functionalized
Undecagold particles only, lyophilized; non-reactive form. 50 nmol
$206
Qty:

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Undecagold Conjugates
Custom Undecagold Conjugates Nanoprobes does not stock undecagold conjugates, but we will be happy to prepare them to order as a custom synthesis. Contact us for a quote
View Cart Paying by purchase order? Please use our online order form.
International orders: Our regional distributors can provide expedited customs processing and delivery.

 

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