Goldiblot His Detection Kit is a rapid, sensitive alternative to enzymatic methods for the detection of recombinant His-tagged proteins on membrane blots such as immunoblots and westerns.
It uses nitrilotriacetic acid (NTA)-nickel (II)-functionalized gold nanoparticles, which bind tightly and selectively to His tags, followed by metal enhancement for direct visualization.
Goldiblot is fast, generating specific magenta bands in one hour with low background, even when the target His-tagged protein is mixed with fifty times as much untagged E coli protein extract.
It is also highly sensitive, capable of detecting down to 2.5 ng (example using His-tagged ATF-1 protein).
The protocol is simple, involving only one detection agent-binding step.
This simplification contributes to the faster speed of the procedure, which can be used to detect quantities of protein in the 25-100 ng range within 45 minutes.
Nickel-NTA Nanogold particles with auto-metallographic amplification provide rapid, sensitive visualization.
High sensitivity: low nanogram-level protein detection in one hour.
Low background and clean signal.
Permanent signal.
Western blot detection of His-tagged proteins using GoldiBlot™ His Western Blot kit (total development time: 1 hour). Lane M: All Blue protein ladder. Lanes 1-5: His-tagged ATF-1 loaded at 2.5 50 ng (1) 50 ng, (2) 25 ng, (3) 10 ng, (4) 5 ng and (5) 2.5 ng. Lane 6: 100 ng His-tagged YY1. Lane 7: 100 ng His-tagged Src. Lane 8: 50 ng His-tagged Src and bacterial extract with 2,500 ng total E. Coli Protein. Lane 9: bacterial extract with 2,500 ng total E. Coli Protein.
Paying by Purchase Order? Please use our Online Order Form. Custom conjugation is also available, of Nanogold®, FluoroNanogold, undecagold
or colloidal gold to primary antibodies, peptides, small molecules, or other molecules.
![[GoldiBlot Western Blot (43k)]](../Images/CatFig_GoldiBlot_1.jpg)