GoldiBlot: Faster His-Tagged Recombinant Protein Detection

Contents

• See HIS-tagged proteins in under an hour!
• Features of GoldiBlot
• Applications
• Ordering and Price List
• References for GoldiBlot

See His-tagged Proteins in under an hour

Goldiblot His Detection Kit is a rapid, sensitive alternative to enzymatic methods for the detection of recombinant His-tagged proteins on membrane blots such as immunoblots and westerns.

It uses nitrilotriacetic acid (NTA)-nickel (II)-functionalized gold nanoparticles, which bind tightly and selectively to His tags, followed by metal enhancement for direct visualization.

• Nickel-NTA Nanogold particles with auto-metallographic amplification provide rapid, sensitive visualization.
• High sensitivity: low nanogram-level protein detection in one hour.
• Low background and clean signal.
• Permanent signal.

Western blot detection of His-tagged proteins using GoldiBlot His Western Blot kit (total development time: 1 hour). Lane M: All Blue protein ladder. Lanes 1-5: His-tagged ATF-1 loaded at 2.5 50 ng (1) 50 ng, (2) 25 ng, (3) 10 ng, (4) 5 ng and (5) 2.5 ng. Lane 6: 100 ng His-tagged YY1. Lane 7: 100 ng His-tagged Src. Lane 8: 50 ng His-tagged Src and bacterial extract with 2,500 ng total E. Coli Protein. Lane 9: bacterial extract with 2,500 ng total E. Coli Protein.