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Technical Help: Undecagold Labeling Reagents


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FAQ

 

See also

  • Guide to Gold Nanoparticle Labeling
    Detailed description of gold nanoparticle labeling, tips and tricks for successful conjugations, isolation of conjugates, and how to calculate labeling.

 


 

Undecagold or Nanogold®

Should I use Undecagold or Nanogold® for my application?

Undecagold will not be visible in a regular transmission electron microscope, so for most applications we recommend Nanogold®.

Undecagold is recommended for use in cryo-electron microscopy, and with high-resolution instruments such as the Scanning Transmission Electron microscope (STEM) or by researchers who would use it as a test for the resolution of their instruments.

Undecagold is useful for very high resolution studies where extensive image analysis is performed, or many images combined to give higher structural resolution than afforded by the instrument directly. For an example, see:

  • Zlotnick, A., Cheng, N., Stahl, S. J., Conway, J. F., Steven, A. C., and Wingfield, P. T.: Localization of the C terminus of the assembly domain of hepatitis B virus capsid protein: Implicatins for morphogenesis and organization of encapsidated RNA; Proc. Natl. Acad. Sci. USA, 94, 9556-9561 (1997).

Undecagold may also be useful for permeation or diffusion studies in which the size of pores or hole structures in biological compartments or membranes are measured. Undecagold has an overall size (including coordinated ligands) of 2.0 nm; Nanogold® has an overall size of 2.5 to 2.7 nm.

 

Catalog Info: Undecagold | Instructions | References


 

Silver Enhancement

Can undecagold be enhanced with silver?

Yes, but much more slowly than Nanogold®. While exact comparisons of the rates of reaction are difficult, immunoblot tests with undecagold-labeled antibodies suggest that the smallest quantities of antigen visulized with un undecagold probe is two to three orders of magnitude more than that visualized with the corresponding Nanogold® probe (i.e. undecagold is two to three orders of magnitude less sensitive).

Reference:

  • Hainfeld, J. F., and Furuya, F. R.: Silver-enhancement of Nanogold and undecagold: in Immunogold-Silver Staining: Principles, Methods and Applications," M. A. Hayat (Ed.); CRC Press, Boca Raton, FL, 1995, pp. 71-96.

 

Catalog Info: Undecagold | Instructions | References


 

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