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Labeled SC35 pre-mRNA Splicing Factor in HeLa Cells


By Fluorescence:

[Fluorescence

Fluorescence micrographs of labeled HeLa cells (A) Stained with monoclonal primary antibody against SC35 pre-mRNA splicing factor, followed by secondary fluorescein and Nanogold-labeled anti-mouse Fab'; (B) Control stained with fluorescein and Nanogold-labeled anti-mouse Fab' only (Final magnification = 1,000 X, bar = 10 m).

By Electron Microscopy:

[Electron

Transmission electron micrographs of labeled HeLa cells. (A) Stained with monoclonal primary antibody against SC35 pre-mRNA splicing factor, followed by secondary fluorescein and Nanogold-labeled anti-mouse Fab'; (B) Control stained with fluorescein and Nanogold-labeled anti-mouse Fab' only (Final magnification = 12,000 X, bar = 1 m).

Reproduced courtesy of The Histochemical Society.

From:

Powell, R. D.; Halsey, Carol M. R.; Spector, D. L.; Kaurin, S. L.; McCann, J., and Hainfeld, J. F.: A covalent fluorescent-gold immunoprobe: "simultaneous" detection of a pre-mRNA splicing factor by light and electron microscopy. J. Histochem. Cytochem., 45, 947-956 (1997).

All micrographs were obtained by Dr. David L. Spector, Shelley L. Kaurin and Jennifer McCann (Cold Spring Harbor Laboratory).


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