Undecagold

Updated: June 1, 2006 Contents Introduction Features of Undecagold Applications Undecagold (Au11) Labeling Reagents: Monomaleido Undecagold Mono-Sulfo-NHS- Undecagold Monoamino Undecagold Charged Undecagold Non-Functional Undecagold Undecagold Conjugates Current Price List Monomaleido Undecagold Monoamino Undecagold Charged Undecagold Mono-Sulfo-NHS-Undecagold Non-functional Undecagold Undecagold labeling reagents are now available in three different packages: 50 nmol for larger labeling experiments, 5 X 10 nmol for several smaller labeling experiments, or in single 10 nmol vials so you can try these reagents on a smaller scale for a low price.

Introduction

Note: Single undecagold clusters are not routinely visualized directly in the TEM. Undecagold may be seen upon image processing of protein helices and crystals, or visualized en masse if there is a bulk deposition such as staining of an organelle. Also, undecagold develops more slowly and with less final silver deposition than Nanogold®. Therefore, for many applications we recommend Nanogold®.

Structure of undecagold showing the [Au₁₁] core, coordinated ligands and peripheral reactive group (maleimide).

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Features of Undecagold

• Ultra small 0.8 nm gold core.
• Use to prepare the smallest possible gold probes.
• Highest possible resolution.
• Site specific covalent labeling with choice of reactivities.

Applications

• Scanning transmission electron microscopy (STEM). [References]
• Labeling of F-actin at the Cys-374 residue by reacting with monomalemido undecagold; data was collected from frozen hydrated samples in the EM and image processing used to map the distribution of label (see: Milligan, R.A., et al.: Nature, 348, 217-221 (1990)). [Other references]
• Labeling of a cysteine in cytochrome oxidase crystals and EM visualization in uranyl acetate/glucose using image processing. [References]
• tRNA labeling with undecagold using a modified nucleic acid base (see: Blechschmidt, B., et al.: J. Struct. Biol., 110, 84-89 (1993)). [Other references]
• Localization of the C terminus of the assembly domain of hepatitis B virus capsid protein by electron microscopy, with image processing (see: Zlotnick, A., et al.: Proc. Natl. Acad. Sci. USA, 94, 9556-9561 (1997)).
• Isomorphous heavy atom replacement in crystallography of large structures by EM crystallography, single particle alignment work, or X-ray diffraction. Provides a signal much greater than a single heavy atom label (see: Thygesen, J., et al. [review]: Structure, 4, 513-518 (1996)). [Other references]
• Labeling carbohydrates on a glycoprotein: sugars are first oxidized to produce aldehydes, which react with the monamino undecagold. [References]

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Undecagold (Au₁₁) Labeling Reagents

Monomaleimido-Undecagold

Applications:

• Create your own small gold probes by linking Au11 to your primary antibody Fab' fragments.
• Label proteins directly for high resolution, site specific identification.
• Label peptides containing cysteine.

Monomaleimido Undecagold Single vial (50 nmol)	Monomaleimido Undecagold Multiple experiments (5 x 10 nmol)	Monomaleimido Undecagold Single vial (10 nmol)
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Product information (PDF)	Product information (PDF)	Product information (PDF)
Material Safety Data Sheet (PDF)	Material Safety Data Sheet (PDF)	Material Safety Data Sheet (PDF)

Catalog / price information: Monomaleido Undecagold

Mono-Sulfo-NHS-Undecagold

Applications:

• Label proteins which do not contain a cysteine.
• Label peptides at the N-terminal.
• Undecagold-label small molecules as inhibitors or substrate analogs for probing transition state structures.

Mono-Sulfo-NHS Undecagold Single vial (50 nmol)	Mono-Sulfo-NHS Undecagold Multiple experiments (5 x 10 nmol)	Mono-Sulfo-NHS Undecagold Single vial (10 nmol)
Instructions and Product information (html)	Instructions and product information (html)	Instructions and product information (html)
Product information (PDF)	Product information (PDF)	Product information (PDF)
Material Safety Data Sheet (PDF)	Material Safety Data Sheet (PDF)	Material Safety Data Sheet (PDF)

Catalog / price information: Mono-Sulfo-NHS-Undecagold

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Monoamino Undecagold

Monoamino Undecagold Single vial (50 nmol)	Monoamino Undecagold Multiple experiments (5 x 10 nmol)	Monoamino Undecagold Single vial (10 nmol)
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Catalog / price information: Monoamino Undecagold

Charged Undecagold

Positively Charged Undecagold	Negatively Charged Undecagold
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Catalog / price information: Charged Undecagold

Non-Functional Undecagold

Applications:

• As a size standard for calibration in high-resolution electron microscopy.
• Sizing pores (see: Schwartz, M. P., and Matouschek, A.: The dimensions of the protein import channels in the outer and inner mitochondrial membranes. Proc. Natl. Acad. Sci. USA, 96, 13086-13090 (1999)). [Complete references]
• For tracing contiguous interior spaces in cells and tissues.

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Catalog / price information: Non-functionalized Undecagold

Undecagold Conjugates

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Price List: Undecagold Reagents

2030A Monomaleimido Undecagold in 5 aliquots (MMUA)
5 x 10 nmol = 50 nmol total: $408
Same total quantity as 2030, but provided in 5 separate tubes. Since the maleimide moiety is unstable once dissolved, separate aliquots are preferable if several smaller quantity labeling reactions are planned at different times.

2030S Monomaleimido Undecagold introductory size (MMUS)
10 nmol: $102
Try Monomaleimido Undecagold on a smaller scale for a low price.

2031 Monoamino Undecagold (MU)
50 nmol: $244
Au₁₁ cluster with a single primary amine. May be used for labeling the carbohydrate moiety of glycoproteins or other uses.

2031A Monoamino Undecagold in 5 aliquots (MUA)
5 x 10 nmol = 50 nmol total: $281
Same total quantity as 2031 but provided in 5 separate tubes.

2031S Monoamino Undecagold introductory size (MUS)
10 nmol: $71
Try Monoamino Undecagold on a smaller scale for a low price.

2043 Positively Charged Undecagold (PU)
50 nmol: $244
Undecagold with a net positive charge; contains amino groups.

2044 Negatively Charged Undecagold (NU)
50 nmol: $244
Undecagold with a net negative charge; contains carboxyl groups. 2045 Mono-Sulfo-NHS-Undecagold (NHSU)
50 nmol: $354
Undecagold with a single N-hydroxy-sulfosuccinimidyl group. Used for labeling primary amines.

2045A Mono-Sulfo-NHS-Undecagold in 5 aliquots (NHSUA)
5 x 10 nmol = 50 nmol total: $408
Same total quantity as 2045 but provided in 5 separate tubes.

2045S Mono-Sulfo-NHS-Undecagold introductory size (NHSUS)
10 nmol: $102
Try Mono-NHS-Undecagold on a smaller scale for a low price.

2060 Undecagold Particles (UG)
50 nmol: $163
Undecagold particles only, concentrated; non-reactive form.

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