Technical Help for Gold Enhancement

Introduction

Other Resources

• Nanoprobes e-Newsletter
  Try our e-mail newsletter for tips and tricks for better gold labeling, and to keep up with new applications.

• Guide to Gold Cluster Labeling
  Detailed description of gold cluster labeling, tips and tricks for successful conjugations, isolation of conjugates, and how to calculate labeling.

• MSA Microscopy Listserver and Archives (view or search).

• Confocal Listserver and Archive (SUNY at Buffalo).

• Tips and Tricks (from the University of Florida ICBR EM core lab).

Nanogold® Conjugates

• Epon embedding
• I can't see any labeling
• Label loss upon osmication
• How can I Lower Background?
• Dual Gold-Silver / DAB Staining
• In Situ Hybridization: No Labeling!

Nanogold® Labeling Reagents

• Can I divide reagent?
• Nucleotide Labeling
• Reaction volumes
• Better Solutions
• How can I Lower Background?

NTA-Ni(II)-Nanogold®

• Reducing background binding
• Eliminating aggregation

AuroVist™

• Toxicity is worse than I expected

FluoroNanogold

• Fluorescence is weak
• Reducing fluorescence background
• Reducing gold/silver background

Undecagold

• Nanogold® or Undecagold?
• Silver Enhancement

Silver Enhancement

• Which silver enhancer should I use?
• Background Signal
• Other makers' probes
• Osmication
• Stains
• Dual Gold-Silver / DAB Staining
• In Situ: No Labeling!

Gold Enhancement

• Can I use gold enhancement?
• I'm seeing a fine, granular background
• Help - development is too fast!

Negative Stains

• How do Negative Stains Work?
• What concentration should I use?

Colloidal Gold

• Fab-3 3 nm Gold Conjugates
• Gold particle and antibody concentrations?
• Can I use it to label my own molecules?
• Are Larger Sizes Available?
• What is the size distribution?