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Updated: May 12, 2002

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Notes:

Correlative Microscopy

  1. Humbel, B. M.; De Jong, M. D. M.; Müller, W. H., and Verkleij, A. J.: Pre-embedding immunolabeling for electron microscopy: An evaluation of permeabilization methods and markers. Microsc. Res. Tech., 42, 43-58 (1998).

  2. Keohane, E. M.; Orr, G. A.;Takvorian, P. M.;Cali, A.; Tanowitz, H. B.; Wittner, M., and Weiss, L. M.: Polar tube proteins of Microsporida of the family Encephalitozoonidae; J. Euk. Microbiol., 46, 1-5 (1999).

  3. [new] (0.1k) Powell, R. D., and Hainfeld, J. F.: Combined Fluorescent and Gold Probes for Microscopic and Morphological Investigations. In Gold and Silver Staining: Techniques in Molecular Morphology, (G. W. Hacker and J. Gu, Eds.), CRC Press, Boca Raton, FL; pp. 107-118 (2002).

  4. Powell, R. D.; Halsey, C. M. R., and Hainfeld, J. F.: Combined fluorescent and gold immunoprobes: Reagents and methods for correlative light and electron microscopy. Microsc. Res. Tech., 42, 2-12 (1998).

    • Jim Hainfeld: Medline search (results will be displayed in a new window).

  5. Powell, R. D.; Halsey, C. M. R.; Gutierrez, E.; Hainfeld, J. F., and Furuya, F. R.: Dual-labeled probes for fluorescence and electron microscopy. Proc. 56th Ann. Mtg., Micros. Soc. Amer.; Bailey, G. W.; Alexander, K. B.; Jerome, W. G.; Bond, M. G., and McCarthy, J. J., Eds.; Springer, New York, NY, 1998, 992-993.

  6. Powell, R. D.; Halsey, C. M. R.; Spector, D. L.; Kaurin, S. L.; McCann, J.;, and Hainfeld, J. F. A covalent fluorescent-gold immunoprobe: "simultaneous" detection of a pre-mRNA splicing factor by light and electron microscopy. J. Histochem. Cytochem., 45, 947-956 (1997).

  7. Powell, R. D.; Hainfeld, J. F.; Halsey, C. M. R.; Spector, D. L.; Kaurin, S.; McCann, J.; Craig, R.; Fay, F. S., and McNamara, K. E.: Large cluster and combined fluorescent and gold immunoprobes. In Proc 54th Ann. Mtg. Micros. Soc. Amer., G. W. Bailey, J. M. Corbett, R. V. W. Dimlich, J. R. Michael and N. J., Zaluzec (Eds.). San Francisco Press, San Francisco, CA, pp. 892-893 (1996).

  8. Powell, R.D., Hainfeld, J.F., Churchill, M.E.A., and Belmont, A.S.I. Combined fluorescent and gold nucleic acid probes. In G. Bailey and A.J. Garratt-Reed (Eds.). Proc 52nd Ann. Mtg. Micros. Soc. Amer., San Francisco Press, pp.176-177 (1994).

  9. Robinson, J. M.; Takizawa, T.: Biological labeling and correlative microscopy; Proc. 57th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 474-475.

  10. Robinson, J. M.; Takizawa, T., and Vandré, D. D.: Applications of gold cluster compounds in immunocytochemistry and correlative microscopy: comparison with colloidal gold. J. Microsc., 199, 163-79 (2000).

  11. Robinson, J. M.; Takizawa, T., and Vandré: Enhanced immunolabeling efficiency using ultrasmall immunogold probes: Immunocytochemistry J. Histochem. Cytochem., 48, 487-492 (2000).

  12. Robinson, J. M.; Takizawa, T.; Vandré, D. D., and Burry, R. W.: Ultrasmall immunogold particles: important probes for immunocytochemistry; Microsc. Res. Tech., 42, 13-23 (1998).

  13. Robinson, J. M.: FluoroNanogold: an efficient labeling reagent for immunocytochemistry. Proc. 56th Ann. Mtg., Micros. Soc. Amer.; Bailey, G. W.; Alexander, K. B.; Jerome, W. G.; Bond, M. G., and McCarthy, J. J., Eds.; Springer, New York, NY, 1998, 990-991.

  14. Robinson, J. M., and Vandré, D. D. Efficient immunocytochemical labeling of leukocyte microtubules with FluoroNanogold: An important tool for correlative microscopy. J. Histochem. Cytochem., 45, 631-642 (1997).

  15. Takeuchi, S.; Takagishi, Y.; Yasui, K.; Murata, Y.; Toyama, J., and Kodama, I.: Voltage-gated K(+)Channel, kv4.2, localizes predominantly to the transverse-axial tubular system of the rat myocyte J. Mol. Cell.Cardiol., 32, 1361-1369 (2000).

  16. Takizawa, T., and Robinson, J. M.: Analysis of antiphotobleaching reagents for use with FluoroNanogold in correlative microscopy J. Histochem. Cytochem., 48, 433-436 (2000).

  17. Takizawa, T., and Robinson, J. M.: FluoroNanogold is a bifunctional immunoprobe for correlative fluorescence and electron microscopy J. Histochem. Cytochem., 48, 481-485 (2000).

  18. Takizawa, T., and Robinson, J. M.: FluoroNanogold as a probe for high resolution correlation between immunofluorescence and electron microscopy; Proc. 57th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 476-477.

  19. Takizawa, T.; Suzuki, K., and Robinson, J. M.: Correlative Microscopy Using FluoroNanogold on Ultrathin Cryosections: Proof of Principle; J. Histochem. Cytochem., 46, 1097-1102 (1998).

A prototype was used in the following publication:
  • Huang, S.; Deerinck, T. J.; Ellisman, M. H., and Spector, D. L.: In vivo analysis of the stability and transport of nuclear poly(A)+ RNA; J. Cell. Biol., 126, 877-899 (1994).

In Situ

  1. Powell, R. D.; Joshi, V. N.; Halsey, C. M. R.; Hainfeld, J. F.; Hacker, G. W.; Hauser-Kronberger, C.; Muss, W. H., and Takvorian, P. M.: Combined Cy3 / Nanogold conjugates for immunocytochemistry and in situ hybridization; Proc. 57th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 478-479.

Silver and Gold Enhancement

  1. Keohane, E. M.; Orr, G. A.;Takvorian, P. M.;Cali, A.; Tanowitz, H. B.; Wittner, M., and Weiss, L. M.: Polar tube proteins of Microsporida of the family Encephalitozoonidae; J. Euk. Microbiol., 46, 1-5 (1999).

  2. [new] (0.1k) Powell, R. D., and Hainfeld, J. F.: Combined Fluorescent and Gold Probes for Microscopic and Morphological Investigations. In Gold and Silver Staining: Techniques in Molecular Morphology, (G. W. Hacker and J. Gu, Eds.), CRC Press, Boca Raton, FL; pp. 107-118 (2002).

  3. Powell, R. D.; Halsey, C. M. R.; Spector, D. L.; Kaurin, S. L.; McCann, J.;, and Hainfeld, J. F. A covalent fluorescent-gold immunoprobe: "simultaneous" detection of a pre-mRNA splicing factor by light and electron microscopy. J. Histochem. Cytochem., 45, 947-956 (1997).

  4. Powell, R. D.; Halsey, C. M. R., and Hainfeld, J. F.: Combined fluorescent and gold immunoprobes: Reagents and methods for correlative light and electron microscopy. Microsc. Res. Tech., 42, 2-12 (1998).

  5. Powell, R. D.; Joshi, V. N.; Halsey, C. M. R.; Hainfeld, J. F.; Hacker, G. W.; Hauser-Kronberger, C.; Muss, W. H., and Takvorian, P. M.: Combined Cy3 / Nanogold conjugates for immunocytochemistry and in situ hybridization; Proc. 57th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag, New York, NY; 1999, 478-479.

  6. Robinson, J. M.; Takizawa, T.; Vandré, D. D., and Burry, R. W.: Ultrasmall immunogold particles: important probes for immunocytochemistry; Microsc. Res. Tech., 42, 13-23 (1998).

  7. Robinson, J. M., and Vandré, D. D. Efficient immunocytochemical labeling of leukocyte microtubules with FluoroNanogold: An important tool for correlative microscopy. J. Histochem. Cytochem., 45, 631-642 (1997).

  8. Takizawa, T., and Robinson, J. M.: FluoroNanogold is a bifunctional immunoprobe for correlative fluorescence and electron microscopy J. Histochem. Cytochem., 48, 481-485 (2000).

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