Ni-NTA-Nanogold®

Updated: June 1, 2006 Contents Introduction Features of Ni-NTA-Nanogold® Current price list Introduction Ni-NTA-Nanogold® is designed for detection or localization of polyhistidine (his) -tagged fusion proteins using electron microscopy, light microscopy or blotting. Using Ni-NTA-Nanogold®, his-tagged fusion proteins originating from any of a variety of expression vectors can be labeled under non-denaturing or denaturing conditions. The labeled his-tagged fusion proteins can be visualized by microscope or blots, when used with gold or silver enhancement reagents such as our Gold Enhance EM (Catalog number 2113), Gold Enhance LM (Catalog number 2112), or HQ Silver (Catalog Number 2012).

Features of Ni-NTA-Nanogold®

• Detects and localizes His-tagged recombinant proteins in electron microscope, light microscope and blots.
• Good product stability.
• 1.8 nm diameter gold particle.
• High resolution for ultrastructural studies.
• Permanent stain does not fade.

Instructions and technical information (html)
  Instructions (PDF)
  Material Safety Data Sheet (PDF)

left: Structure of Ni-NTA-Nanogold® showing interaction with Interaction with a His-tagged protein; right: Knob protein from adenovirus cloned with 6x-His tag, labeled with Ni-NTA-Nanogold, column purified from excess gold, and viewed in the scanning transmission electron microscope (STEM) unstained (Full width approximately 245 nm).

More information:

• See Our 2002 paper on Ni-NTA-Nanogold®.
• References for Ni-NTA-Nanogold®
• Technical Help for Ni-NTA-Nanogold®

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Price List: Ni-NTA-Nanogold®

Supplied as an aqueous solution in 3 mL of 50 mM MOPS, pH 7.9.

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