Ni NTA-Nanogold® binds directly to His-tags
No antibodies or special equipment needed
Fast. Simple. Sensitive. Archival.
GoldiBlot™ uses our unique His-tag targeting gold nanoparticle probe, Ni-NTA-Nanogold®, combined with fast, clean signal amplification using our autometallographic developer, GoldEnhance™.
- Economical: No expensive primary or secondary antibodies, or chemiluminescent or chromogenic substrates. Cost per blot is only about $22 (USD; based on 10 mL reagent volume per blot, or about one 7 x 8.4 cm blot).
- Fast: Complete in about an hour, vs ~3 hours with antibodies.
- Universal: Detect His tags at any position: C-terminal, N-terminal, even internal. Tiny nanoparticles penetrate better, labeling where antibodies can fail.
- Sensitive: Get strong, specific binding at low concentrations for clear, sensitive visualization. The Nickel NTA interaction is as strong as antibody-antigen binding (binding constant ~1013 M-1).
- Reliable: Uses a simple reaction without the variability of anti Histidine tag antibodies.
- Highly Specific: Ni NTA Nanogold® nanoparticles have similar binding capabilities to IMAC, and specifically detect polyhistidine tags without any requirement for specific adjacent groups.
- Direct Visualization: No chemiluminescent substrates, X-ray film or CCD imagers.
- Stable: All reagents are highly stable, with shelf life longer than three years.
- Archival: Long-lived, high contrast– Dark purple-black staining is stable and does not fade, making imaging and archiving straightforward.
The GoldiBlot™ process: Ni-NTA-Nanogold® binds to a polyhistidine (His) tag, then gold enhancement generates visible signal.
Check out all our His-tag detection kits:
10 nm Ni-NTA-Nanogold®
Optimum size for direct EM visualization
Use for post-embedding labeling
5 nm Ni-NTA-Nanogold®
Go straight to EM-
No silver / gold enhancement needed!
1.8 nm Ni-NTA-Nanogold®
Great for cryo-EM
Smallest probe: best penetration and highest resolution
Fast and simple procedure
Sensitive and reliable results
GoldiBlot™ procedure and an example of the results, showing detection of His-tagged proteins from a variety of samples.
How do we compare? See for yourself.
Compare Time and Cost
Labeling & detection method
Time to complete visualization
Cost per blot (USD)
GoldiBlot™ His-tag Western Blot Kit
1 hour 15 mins
$ 22
Chromogenic detection: peroxidase-labeled anti-His tag antibody and metal-enhanced DAB substrate
2 hours 50 minutes
$ 35
Chemiluminescent detection: peroxidase-labeled anti-His tag antibody and chemiluminescent HRP substrate
2 hours 50 minutes
$ 45
From our scientists:
An Introduction to GoldiBlot™
Ni NTA-Nanogold® kits for His tag Blots
with Dr. Richard Powell, PhD
Our video introduction explains how Ni-NTA-Nanogold® works and how it compares with other methods for detecting His tags.