Undecagold Labeling Reagents

Product Catalog Nanogold® Labeling Reagents Recombinant Protein Detection   Ni-NTA-Nanogold® GoldiBlot™ Nanogold® Conjugates AuroVist™ FluoroNanogold™ Gold Lipids Silver Enhancement GoldEnhance EnzMet™ Negative Staining Conducting Polymers Undecagold Custom Synthesis Colloidal Gold Order Form (for Purchase Orders) Current Price List (pdf) Ordering Information

Contents Introduction Features of Undecagold Applications Undecagold (Au11) Labeling Reagents: Monomaleido Undecagold Mono-Sulfo-NHS- Undecagold Monoamino Undecagold Charged Undecagold Non-Functional Undecagold Undecagold Conjugates Complete List of References Current Price List Monomaleido Undecagold Monoamino Undecagold Charged Undecagold Mono-Sulfo-NHS-Undecagold Non-functional Undecagold Technical Help for Undecagold Reagents Undecagold labeling reagents are now available in three different packages: 50 nmol for larger labeling experiments, 5 X 10 nmol for several smaller labeling experiments, or in single 10 nmol vials so you can try these reagents on a smaller scale for a low price.

Introduction

Note: Single undecagold clusters are not routinely visualized directly in the TEM. Undecagold may be seen upon image processing of protein helices and crystals, or visualized en masse if there is a bulk deposition such as staining of an organelle. Also, undecagold develops more slowly and with less final silver deposition than Nanogold®. Therefore, for many applications we recommend Nanogold®.

Structure of undecagold showing the [Au₁₁] core, coordinated ligands and peripheral reactive group (maleimide).

Technical Help for Undecagold Reagents
References for Undecagold
Undecagold References by application
Top of page

Features of Undecagold

• Ultra small 0.8 nm gold core.
• Use to prepare the smallest possible gold probes.
• Highest possible resolution.
• Site specific covalent labeling with choice of reactivities.

Applications

• Scanning transmission electron microscopy (STEM). [References]
• Labeling of F-actin at the Cys-374 residue by reacting with monomalemido undecagold; data was collected from frozen hydrated samples in the EM and image processing used to map the distribution of label (see: Milligan, R.A., et al.: Nature, 348, 217-221 (1990)). [Other references]
• Labeling of a cysteine in cytochrome oxidase crystals and EM visualization in uranyl acetate/glucose using image processing. [References]
• tRNA labeling with undecagold using a modified nucleic acid base (see: Blechschmidt, B., et al.: J. Struct. Biol., 110, 84-89 (1993)). [Other references]
• Localization of the C terminus of the assembly domain of hepatitis B virus capsid protein by electron microscopy, with image processing (see: Zlotnick, A., et al.: Proc. Natl. Acad. Sci. USA, 94, 9556-9561 (1997)).
• Isomorphous heavy atom replacement in crystallography of large structures by EM crystallography, single particle alignment work, or X-ray diffraction. Provides a signal much greater than a single heavy atom label (see: Thygesen, J., et al. [review]: Structure, 4, 513-518 (1996)). [Other references]
• Labeling carbohydrates on a glycoprotein: sugars are first oxidized to produce aldehydes, which react with the monamino undecagold. [References]

Technical Help for Undecagold Reagents
Complete List of References
Undecagold References by application
Top of page

Monomaleimido-Undecagold

Applications:

• Create your own small gold probes by linking Au11 to your primary antibody Fab' fragments.
• Label proteins directly for high resolution, site specific identification.
• Label peptides containing cysteine.

Monomaleimido Undecagold Single vial (50 nmol)	Monomaleimido Undecagold Multiple experiments (5 x 10 nmol)	Monomaleimido Undecagold Single vial (10 nmol)
Instructions and Product information (html)	Instructions and product information (html)	Instructions and product information (html)
Product information (PDF)	Product information (PDF)	Product information (PDF)
Material Safety Data Sheet (PDF)	Material Safety Data Sheet (PDF)	Material Safety Data Sheet (PDF)

Mono-Sulfo-NHS-Undecagold

Applications:

• Label proteins which do not contain a cysteine.
• Label peptides at the N-terminal.
• Undecagold-label small molecules as inhibitors or substrate analogs for probing transition state structures.

Mono-Sulfo-NHS Undecagold Single vial (50 nmol)	Mono-Sulfo-NHS Undecagold Multiple experiments (5 x 10 nmol)	Mono-Sulfo-NHS Undecagold Single vial (10 nmol)
Instructions and Product information (html)	Instructions and product information (html)	Instructions and product information (html)
Product information (PDF)	Product information (PDF)	Product information (PDF)
Material Safety Data Sheet (PDF)	Material Safety Data Sheet (PDF)	Material Safety Data Sheet (PDF)

Monoamino Undecagold

Monoamino Undecagold Single vial (50 nmol)	Monoamino Undecagold Multiple experiments (5 x 10 nmol)	Monoamino Undecagold Single vial (10 nmol)
Instructions and Product information (html)	Instructions and product information (html)	Instructions and product information (html)
Product information (PDF)	Product information (PDF)	Product information (PDF)
Material Safety Data Sheet (PDF)	Material Safety Data Sheet (PDF)	Material Safety Data Sheet (PDF)

Charged Undecagold

Positively Charged Undecagold	Negatively Charged Undecagold
Instructions and Product information (html)	Instructions and product information (html)
Product information (PDF)	Product information (PDF)
Material Safety Data Sheet (PDF)	Material Safety Data Sheet (PDF)

Non-Functionalized Undecagold

Applications:

• As a size standard for calibration in high-resolution electron microscopy.
• Sizing pores (see: Schwartz, M. P., and Matouschek, A.: The dimensions of the protein import channels in the outer and inner mitochondrial membranes. Proc. Natl. Acad. Sci. USA, 96, 13086-13090 (1999)). [Complete references]
• For tracing contiguous interior spaces in cells and tissues.

Instructions and technical information (html)
  Instructions (PDF)
  Material Safety Data Sheet (PDF)

Undecagold Conjugates

Nanoprobes does not stock undecagold conjugates, but we will be glad to prepare them to order as a custom synthesis. Let us know your needs, and we'll give you a quote: Use our online form, e-mail us at tech@nanoprobes.com, or call us at 877-447-6266 (US toll-free number) or (631) 205-9490. We look forward to working with you!

Top of page

Top of page